Remission of Type 2 Diabetes and Decreased Inflammation and Cardiovascular Risk Factors in Obese Adults Using a High Protein Diet: Randomized Control Trial

Frankie B. Stentz ¹_, Damon Lawson¹_, Sidney Tucker¹, John Christman¹_, Chris Sands¹

¹Departments of Medicine, Endocrinology, Diabetes and Metabolism Division. The University of Tennessee Health Science Center, Memphis, Tennessee 

*Corresponding Author: Frankie B. Stentz, Departments of Medicine, Endocrinology, Diabetes and Metabolism Division. The University of Tennessee Health Science Center, Memphis, Tennessee 

 Citation: : Frankie B. Stentz (2022) Remission of Type 2 Diabetes and Decreased Inflammation and Cardiovascular Risk Factors in Obese Adults Using a High Protein Diet: Randomized Control Trial1(1).

Copyright: @ 2022 Frankie B. Stentz, This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Received: 06 October 2022| Accepted:12 November 2022|Published: 24 November 2022

Keywords: type 2 Diabetes remission, insulin sensitivity, inflammation, high protein diet, weight loss, cardiovascular risk factors

Abstract

Objective: Remission of Type 2 Diabetes (T2D) to Normal Glucose Tolerance (NGT) is an important health concern which has had little success in the past. This study’s objectives were to determine the effect on remission of T2D with a high protein(HP) vs. high carbohydrate(HC) diet and effects on inflammation, metabolic parameters, oxidative stress, cardiovascular risk factors(CVR), lean and fat body mass in obese subjects with T2D after 6 months of dietary intervention.

Research Design and Methods: We recruited and randomized 12 women and men with T2D to either a HP(30%protein, 30%fat, 40%carbohydrate)(n=6) or HC(15%protein, 30%fat, 55%carbohydrate)(n=6) diet feeding study for 6 months in this randomized controlled trial. All meals were purchased at local grocery stores and provided to subjects for 6 months with daily food menus for HP or HC compliance with weekly food pick-up and weight measurements. Oral glucose tolerance and meal tolerance tests with glucose and insulin measurements and DXA scans were done at baseline and after 6 months on the respective diets.

Results: After 6 months on the HP diet, 100% of the subjects had remission of their T2D to NGT, whereas only 16.6% of subjects on the HC diet had remission of their T2D. The HP diet group exhibited significant improvement in a)insulin sensitivity(p=0.001), b) inflammatory cytokines(p=0.001), c)cardiovascular risk factors(p=0.004), d)oxidative stress(p=0.001), e)increased %lean body mass(p=0.001) compared to the HC diet group at 6 months.

Conclusions: 100% remission of T2D to NGT with a HP diet and significant improvement in inflammation, metabolic parameters and cardiovascular risk factors was achieved compared to a HC diet at 6 months. 

Introduction

  The prevalence of people with Type 2 Diabetes Mellitus (T2DM) has continued to increase over the past 20 years with currently approximately 37 million people with diabetes, 27 million who are diagnosed and 7.2 million who are undiagnosed in the USA according to the Center for Disease Control(CDC) 2022 National Diabetes Statistics Report (1).  Obesity is one of the highest risk factors for T2DM, as well as for, heart disease, hypertension, and other metabolic disease (2).It  has reached epidemic proportion in the U.S. with over 42% adults estimated to be obese(3,4). As Body Mass Index (BMI) increases from 23 to > 35 kg/m² there is 93 fold increase in T2DM [4, 5]. The Diabetes Prevention Program (DPP)(6,7)and ACT NOW (8) studies showed that the rate of conversion for Impaired Glucose Tolerance (IGT)(prediabetes) to T2DM is between 7-10%/year with no significant difference in ethnicity.  Subjects with diabetes are at increased risk of numerous medical and complications such as seen in COVID-19 subjects with diabetes have had a higher incidence of hospitalization and mortalit(9,11).   DPP (6,12) and other similar diabetes prevention studies(13,14)  have shown the importance of diet in reducing the risk of conversion of IGT to T2DM. Attempts to reduce the risk of T2DM, complications and medical costs must start in the early part of diagnosis(15,16). Although numerous diets have been recommended for T2DM (18,19) and non-diabetics, and discussed the potential advantages of low-carbohydrate (23,24)or high-protein diets(25,26), there is no consensus on a specific weight loss diet to manage blood glucose in T2DM(27).   Our studies of the effect of a High Protein (HP) diet or High Carbohydrate (HC) diet on IGT obese subjects (28) and NGT obese subjects (29) demonstrated similar weight loss of 9-10% in both diet interventions, butgreater advantages of the HP diet for insulin sensitivity (100% remission of IGT to NGT), reduced CVR factors, oxidative stress (ROS), and inflammatory cytokines(IC)(28,29). HP intake has the potential to suppress hunger and induce satiety (18,25,30) a negative relationship between protein content and glycemic index(31,32). Increased protein intake has also been shown to reduce energy intake independent of the effect of satiety(25). Proteins also increase the thermic effect of feeding (33) mostly by increasing protein synthesis. Lipids are considered a primary risk factor for CV disease and dietary composition can affect the lipid profile and its metabolism(34,37). Our studies showed a greater decrease in TG in the HP diet (28,29) demonstrating that increasing proteins in the diet may alter the lipid profile in a beneficial way.  It has been shown that protein intake by itself induces insulin release, is different in diabetic and non-diabetic individuals (38) and protein is a much less potent secretagogue for insulin than is glucose in normal individuals(39). Our studies showed a lower insulin response to the HP than the HC diet(28,29) This suggests that HP diets could help preserve the Beta cells by increasing sensitivity and decreasing insulin load per meal. Our studies on the incretin response to HP and HC diets showed an increased release in GLP-1 and GIP with the HP diet compared to the HC diet(40,41). Treatment of T2DM subjects with GLP-1 receptor agonists and DPP-4 inhibitors have been found to improve insulin sensitivity and cardiovascular risks(27,42,48). These studies suggest that our HP diet may be beneficial in treating T2DM and have cardiovascular benefits.

Other important aspects of the HP diet are the decreased glucose area under the curve (AUC) and the anti-inflammatory effect compared to the HC diet (28,29). Studies have demonstrated that hyperglycemia or elevation of Free Fatty Acids (FFA) lead to activation of leukocytes and increase in cytokines and reactive oxygen species (ROS)(49,50). Studies have shown that hyperglycemia in T2DM and obesity is associated with increased inflammatory cytokines(53,54). Thus, reduction in inflammatory cytokines in diabetes is important in view of the fact that patients who develop acute respiratory distress (55,58) and COVID-19 have increased inflammatory cytokines, and diabetes would add to this inflammatory effect(59).

Protein content in the diet may also help maintain lean body mass(28,60,61).  Few studies have compared diets with adequate percentages of macronutrients and sufficient follow-up time where high protein is compared to high carbohydrate diets and determined effects on CVR factors, ROS, inflammatory cytokines (28, 29, 52, 53, 62-66) especially with respect to subjects with Type 2 Diabetes.

 Since there has not been established a diet for weight loss and glucose control and converting from T2DM to Normal Glucose Tolerance (NGT) and given the destructive metabolic changes that occur with T2DM it would be prudent to determine if one exists. Therefore, this study was designed to determine if remission of type 2 diabetes in newly diagnosed type 2 diabetes patients could be obtained with a tightly controlled HP or HC diet feeding study and quantitate longitudinally the changes in various metabolic markers of insulin sensitivity, inflammatory cytokines, CVR factors, ROS, and changes in muscle and fat mass and weight loss from baseline to 6 months.

RESEARCH DESIGN AND METHODS

PATIENTS

      Women and men age 20-60 years old with a BMI > 30 kg/m² to < 55 kg/m² diagnosed with type 2 diabetes with the past two years or diagnosed at this study screening visit were recruited for the study. Subjects were selected on the basis of inclusion criteria of age, BMI, fasting glucose of >126 mg/dl, 2 hour glucose level of  >200 mg/dl during a standard oral glucose tolerance test, and HbA1c of 6.5-10%. They were excluded if  they had proteinuria or elevated serum creatinine(> 1.5 mg/dl), history of liver disease, abnormal liver function tests,  on antidiabetic agents or insulin, thyroid disease with abnormal TSH, weight > 350 lbs, triglycerides > 400 mg/dl, LDL cholesterol > 160 mg/dl, SBP > 145 or DBP > 100 mm, use of medications known to effect glucose or lipid metabolism, pregnancy or the desire to become pregnant in the next 6 months, weight loss of more than 5% of body weight in the last 6 months, smoked, or history of cancer undergoing active treatment. Subjects who were on metformin who wanted to participate in the study were discontinued from the drug by their PCP and baseline tests were performed on these subjects 4 weeks after discontinuing the drug. Subjects that met the above criteria were asked to keep a food diary for a week. Those found to be non-adherent and unable to keep diet diary or deemed unable to adhere to the protocol were excluded from the study.

            Of the 83 subjects screened by phone 26 were asked to come in to sign the consent form and testing for meeting the inclusion criteria. Fifteen of these subjects met all the inclusion criteria and were randomized to a HP diet (7 subject) vs. HC diet (8 subjects) for a period of 6 months. One subject in the HP group and 2 subjects in the HC group dropped out within a few weeks after screening due to their work schedule, driving distance or moved out of area.  Since there was no data (OGTT, MTT, DXA, weight, metabolic markers) other than baseline on those subjects that dropped out they were not included in the data analysis of comparison of changes from baseline to 6 months on the diet interventions. Therefore, six subjects in each group completed the 6 month study as shown in Figure 1 and data analyzed.

Figure 1. Shows the recruiting and screening of subjects for the participants in the study.

STUDY DESIGN

The study was a prospective randomized trial of a High Protein (HP) diet (30% Kcals from protein, 40% Kcals from carbohydrate (CHO), 30% Kcals from fat) vs High Carbohydrate (HC) diet (15% Kcals from protein, 55% Kcals from CHO, 30% Kcals from fat) for a period of 6 months. The study was approved by the Institutional Review Board of the University of Tennessee Health Science Center (UTHSC)

All participants were seen in the General Clinical Research Center (GCRC) at UTHSC for all their visits.  After signing the consent form, a history and physical examination, height and weight, blood pressure and waist measurements were done.  At the baseline and at 6 months of the study participants underwent a standard OGTT and mixed meal tolerance test (MMT). The meal for the MTT for the HP group was a high protein meal and the meal for the MTT for the HC group was a HC meal. Both HP and HC meals were 300 calories (the same as the 75 gm OGTT).  Each provocative test was done after an overnight fast with two or more days between tests. Glucose and insulin were measured at baseline and at 30 minute intervals for 2 hours.  These tests were repeated after being on the assigned diet for 6 months.  Dual energy x-ray absorptiometry (DXA) scan, resting metabolic rate (RMR), chemistry profile, Complete Blood Count(CBC), vitamin D, Parathyroid Hormone(PTH),  lipid profiles, as well as 24 hour urine collections for creatinine clearance (CrCl), microalbumin, calcium(Ca) and urinary urea nitrogen (UUN) were all done at baseline and 6 months..  These determinations were done to determine insulin sensitivity and glucose response, lipid profile, changes in body weight and body composition (lean and fat mass), calcium metabolism and protein breakdown (by urinalysis). Subjects were assessed for level of activity and all were at minimum activity. Per the American Diabetes Association (27) recommendation of 150 minutes of exercise per week, patients were asked to walk 30 minutes a day and were given FitBits to monitor their level of physical activity which was monitored weekly throughout the study. After meeting the screening criteria subjects were randomized to either the HP or HC diet using a permuted block randomization method generated by the biostatistician.

Subjects were considered to have remission of their Type 2 Diabetes to normal glucose tolerance (NGT) if at 6 months they had a fasting glucose of  <100 mg/dl, and a  2 hour glucose level of  < 140  mg/dl during a single oral glucose tolerance test and HbA1c < 5.7 %.  They were considered to have remission to prediabetes if at 6 months they had a fasting glucose of  100  to <126 mg/dl, and a  2 hour glucose level of  140 to 199  mg/dl during the OGTT and HbA1c >5.7- 6.4%. Subjects who at the 6 month OGTT had a fasting glucose of  >126 mg/dl and a  2 hour glucose level of  >200  mg/dl and HbA1c > 6.5% did not have remission of their Type 2 Diabetes and were referred back to their Primary Care Physician (PCP) or Endocrinologist for pharmaceutical treatment. Subjects who had remission of Type 2 Diabetes were transitioned to purchasing their own food and meal preparations using the diet plans they were using during the study when all food was provided. These subjects were followed for an additional 6 months by phone, emails and dietary consultation to help them maintain their remission of diabetes and weight loss.

DIET RELATED PAREMETERS:

 Diet Composition:

 Study participants were randomized using a randomization table to either the HP or HC diet for 6 months. Calorie maintenance needs were determined on an individual basis using their resting metabolic rate (RMR). After maintenance caloric needs were established for each individual, 500 Kcals/day were subtracted from the determined caloric needs to promote a 1-2 lbs weekly weight loss. On average, a 1700 Kcal/day diet for a 100 kg individual was used to achieve adequate weight loss.  A one-time calorie reduction of 200 Kcal was made if a subject reached a plateau and did not lose weight for two consecutive weeks during the 6 months.  No subject was on less than 1200 Kcal/day.

     A feeding study where all food and daily menus were provided was necessary to ensure accurate macronutrient consumption. The meals were dispensed as pre-packaged foods from stored frozen food by the dietician affiliated with the UT CRC dietary services on the site of the CRC, maintaining the macronutrient and caloric requirements established at randomization. Subjects were given 3 meals a day plus snacks between meals. All foods were available at local grocery stores and met the recommended daily intake of vitamins and mineral for women and men age 20-60 years as assessed by the University of Minnesota Nutrition Data system. Both the HP and HC diets contained more than the recommended daily intake (RDI) for calcium of 1000 mg/day for women and men 20-60 years of age [67] by providing an average of 1500 mg Ca for both diets.  As the participants were obese with Type 2 Diabetes and might be at risk for coronary heart disease, it was prudent to ensure the participants follow a healthy diet that would minimize their health risks. For example, dietary fat sources focused on monounsaturated and polyunsaturated fats, i.e., plant oils, semi-liquid margarine, nuts (63,68,69). Dietary carbohydrate sources emphasized whole grains, fruits, vegetables and legumes; and dietary protein sources included lean meats, fish, chicken, eggs and non-fat dairy foods, i.e. fat-free milk and low-fat cheese. The dietary principles are consistent with the guidelines of the Institute of Medicine[68] and American Diabetes Association(27).

Diet Compliance

                       Participants for this feeding study were given detailed instructions during the pre-study orientation at screening visits. A variety of foods with the same macronutrients were offered to increase choices and adherence. After choosing their preferences for meals and snacks, participants were instructed to adhere to the diet to which they were assigned. All food was provided for the entire 6 months to the participants who came in weekly to pick up their food and daily menu food record for the week and weight measurements. Participants were required to turn in their record when they returned for the next food pick up to check for compliance of diet adherence.

Frequent interaction, behavior modification, individualized diet with food variety and food record systems which have been shown to increase dietary compliance were used. Food records served as a “motivational enforcement” and recording dietary intake emphasized the importance of the diet as a key component to the study (70). Compliance was assessed by objective and subjective parameters which included weekly subject contact and detailed review of their food diaries.

Assessment of Body Composition by DXA:

 Hologic Discovery QDR Bone Densitometer (version 8.3) was used to measure body-composition by measurements of the entire body. DXA measurements were done at baseline and 6 months. Body composition including lean mass (LM), fat mass (FM), and bone mineral content (BMD) were assessed(28).  The DXA Quality Assurance manual for the UTHSC Clinical Research Center (CRC) was used to standardize patient positioning and scan analysis.

Resting Metabolic Rate (RMR):

Indirect calorimetry was used to assess RMR of each participant at the beginning of the study to determine the caloric diet intake needs for weight loss for each subject and at 6 months to determine changes in their metabolic rate.  A Cardio Coach(Korr Medical Technologies) was used for the determination of the RMR.  The subjects after an overnight (10 hr) fast were at absolute rest in a reclining position for 15 minutes after which respiratory exchanges were measured continuously for 15 min measuring oxygen consumption and CO₂  production to determine the RMR(28,71).

LABORATORY PROCEDURES:  

Determination of Plasma Metabolic Hormones, Cytokines, Markers of Cardiovascular Risks and Lipids.

               Glucose and insulin levels for the OGTT and MTT for baseline and 6 months, were measured at 0, 30, 60, 90, and 120 minutes and AUC calculated using the Trapezoidal rule.  Insulin, glucose, inflammatory cytokines (TNFa, IL-1β, IL-6, INFƔ, MCP-1), CVR factors (blood pressure, triglycerides, LDL, HDL, cholesterol, BMI, hcCRP, FFA), adiponectin, oxidative stress (ROS) (dichlorofluorescein(DCF) and MDA), β-hydroxybutyrate, HbA1c were measured using our previously established methods (28, 29, 53, 57, 58, 72). The Coefficient of Variation of the assays were all less than 5 percent.

Chemistry Metabolic Profile (CMP), CBC with diff, TSH, cortisol, Urine urea nitrogen (UUN) and other tests to exclude chemical and metabolic abnormalities and protein balance were determined by standard clinical lab procedures. Protein and muscle mass catabolism were assessed via 24-hour UUN, CrCl and Ca at baseline and at 6 months. Calcium balance was assessed by 24-hour urine Ca excretion as well as serum Ca, PTH, and 25 OH-vitamin D at baseline and 6 months. BMI and waist were measured by standard methods.

 Insulin Sensitivity and Beta Cell Function

                       The homeostasis model assessment was used to determine the insulin resistance HOMA IR (73).  Insulin sensitivity (ISI) was determined from plasma insulin and glucose measurements from the OGTT by the Matsuda insulin index(8, 74).  Beta cell function were calculated as previously described from plasma glucose and insulin measurements obtained during the 2 hour OGTT (8, 28, 29).

 STATISTICAL ANALYSIS

The primary outcomes examined were remission of Type 2 Diabetes, markers of insulin sensitivity, inflammatory cytokines, cardiovascular risk factors, and change in lean and fat body mass from baseline to 6 months. Initially, change was compared between the two HP and HC diets using Wilcoxon Rank Sum test to compare the effects of the two diets. In addition, Wilcoxon Signed Rank test was used to compare baseline and 6 months data to assess effects of each diet.

To assess the effectiveness of randomization, Wilcoxon Rank Sum test was used to compare baseline variables between the two arms. A p-value less than 0.05 was considered statistically significant. If important baseline differences were identified, they were included in analysis using generalized linear models.

All analyses were conducted using SAS 9.3 (SAS Institute Inc., Cary, NC). Results are presented as mean±SE. Statistical significance was declared if the two-sided p-value was less than 0.05. The study was designed to recruit 6 subjects in each arm. Power analysis was done according to two scenarios at 5% significance level. In the first objective, the changes were compared between the two arms. Assuming the baseline to follow-up correlation is 0.25 and the effect size (the ratio of variance of interaction effects to within cell variance) is 0.40, interaction effects could be tested with at least 80% power. Just as important, in the absence of interaction effects, focus was shifted on assessing marginal treatment arm differences. Assuming again the baseline-to follow-up correlation is 0.25 and an effect size of 0.80 (ratio of mean weight change to within SD), the changes could be tested with at least 80% power. Note that extended models having baseline covariates should lead to greater statistical power under both scenarios.

RESULTS
At baseline the HP and HC groups were not statistically different. Table 1 shows the mean ± SE of various parameters monitored on the twelve (6 HP and 6 HC) diet subjects from baseline through 6 months and the significant difference of changes of the parameters in the subjects on the HP and HC diets.  Significantly important is the 100% (6/6) remission of Type 2 Diabetes to normal glucose tolerance (NGT) in all the HP diet group subjects; whereas, there was only a 16% (1/6) remission to NGT and (1/6) remission to IGT in the HC group. Subjects on both the HP and HC had significant weight loss at 6 months from their baseline weights but not significantly different between the HP and HC groups at 6 months. The waist measurements decreased from 112.5 ± 3 cm at baseline to 105.2 ± 2 cm at 6 months (p=0.001) in the HP diet group and in HC diet group decreased from 110.7 ± 3.4 at baseline to 103.5 ± 4 at 6 months (p=0.005).  HbA1c was significantly improved to normal range with the HP group whereas, the average HbA1c did not decrease below the criteria level of T2D in the HC diet group after 6 months. Insulin sensitivity (HOMA IR and ISI) as shown in Table 1 and Beta cell function which increased from baseline ( 3.1 ± 0.3) to 6 months(11.1± 2.1) (p=0.001) in the HP diet group and from baseline ( 3.2 ± 0.3) to 6 months (5.26± 0.9) (p=0.03) in the HC group were all significantly improved at 6 months from baseline in both the HP and HC diets; however, the HP group had significantly greater improvement in these parameters compared to the HC group at 6 months (p=0.001).  Diet compliance was 94.7% and 94.1% for the HP and HC diet groups, respectively, which were not significantly different.

Table 1. Changes in Insulin Sensitivity and Metabolic Parameters with the HP and HC Diets

ANALYSIS   Mean + SE were calculated 

 **Wilcoxon Rank Sum Test was used to compare variables between the two diet groups at 6 mo  and

*Wilcoxon Signed Rank Test to compare baseline and 6 mo within diet group.  p < 0.05 was considered statistically significant

Table 2 shows the inflammation markers (TNFα, IL-1β, IL-6, IFNƔ, MCP-1, hsCRP) and CVR factors (BP, cholesterol, triglycerides, LDL, HDL), and oxidative stress markers (ROS-DCF, MDA) were significantly decreased in both diet groups. The FFA were significantly decreased in the HP diet group at 6 months but not in the HC diet group. The HP diet resulted in significantly greater reduction in these inflammatory cytokines, CVR factors and oxidative stress markers compared to the HC diet at 6 months.  This reduction in TNFα, IL-1β, IL-6, IFNƔ, MCP-1, and hsCRP demonstrates a better anti-inflammatory effect of the HP diet compared to the HC diet.

Table 2. Changes in Inflammatory, CVR and Oxidative Stress Markers with the HP and HC Diet

ANALYSIS   Mean + SE were calculated 

 **Wilcoxon Rank Sum Test was used to compare variables between the two diet groups at 6 mo  and

*Wilcoxon Signed Rank Test to compare baseline and 6 mo within diet group.  p < 0.05 was considered statistically significant

Figures 2 A shows the mean ± SD for glucose values for the OGTT for the HP and HC diet groups.  There was no significant difference of the baseline (Bl) HP vs HC glucose response as shown by the figure and area under the curve (AUC) for the OGTTs.  OGTT glucose response and AUCs for the HP and HC diet groups were significantly less at 6 months(mo) than at Bl (HP Bl vs HP 6 mo (glucose p=0.0005), HC Bl vs HC 6 mo (glucose p=0.01).  However, the OGTT glucose response AUCs for the HP diet were significantly less than the HC diet at 6 months (p=0.0001) showing a greater improvement in glucose disposal with the HP diet than the HC diet.

Figure 2A: Shows the mean ± SE  of glucose and insulin for the 2 hour OGTTs and MTTs for the 6 HP diet subjects and the 6 HC diets subjects.  The colored lines represent the following: blue line is  HP diet baseline (HP_bl);   red line is HP diet at 6 months (HP_6m);   green line is HC diet baseline (HC_bl); and yellow line is HC diet at 6 months (HC_6m).

Figure 2 B shows the mean ± SD for glucose for the MTT for the HP and HC diet groups. The MTT HP vs HC glucose AUCs at Bl were significantly different (p=0.001) demonstrating the difference in glycemic response to the HP vs HC meal of the same caloric intake (300kcal).  Glucose AUCs for the HP (p=0.005) and HC (p=0.01) diets at 6 months were significantly less than at Bl. However, the glucose AUCs for the HP MTT was significantly less than the glucose of the HC MTT (p=0.0001) after 6 months on the respective diets. Thus, the HP diet caused a decreased blood glucose level, greater glucose disposal and improved insulin sensitivity.

Figure 2B : Shows the mean ± SE  of glucose and insulin for the 2 hour OGTTs and MTTs for the 6 HP diet subjects and the 6 HC diets subjects.  The colored lines represent the following: blue line is  HP diet baseline (HP_bl);   red line is HP diet at 6 months (HP_6m);   green line is HC diet baseline (HC_bl); and yellow line is HC diet at 6 months (HC_6m).

Figures 2C shows the mean ± SD for the insulin values for the OGTT for the HP and HC diet groups.  There was no significant difference of the baseline (Bl) HP vs HC insulin response to the glucose as can be seen by the figure and area under the curve (AUC) for the insulin.  Insulin response and AUCs for the HP and HC diet groups were significantly less at 6 months (mo) than at Bl (HP Bl vs HP 6 mo (p=0.0005), HC Bl vs HC 6 mo (p=0.01). The OGTT insulin AUC response for the HP diet was significantly less than the HC diet at 6 months (p=0.0001) showing a greater improvement in insulin sensitivity with the HP diet than the HC diet

Figure 2C: Shows the mean ± SE  of glucose and insulin for the 2 hour OGTTs and MTTs for the 6 HP diet subjects and the 6 HC diets subjects.  The colored lines represent the following: blue line is  HP diet baseline (HP_bl);   red line is HP diet at 6 months (HP_6m);   green line is HC diet baseline (HC_bl); and yellow line is HC diet at 6 months (HC_6m).

Figure 2 D shows the mean ± SD for insulin for the MTT for the HP and HC diet groups. The MTT HP vs HC insulin AUCs at Bl were significantly different (p=0.001) demonstrating the difference in insulin response to a high protein vs high carbohydrate meal of the same caloric intake (300kcal).  Insulin AUCs for the diets at 6 months were significantly less than at Bl (HP (p=0.001) and HC (p=0.01)) and the insulin AUCs for the HP MTT were significantly less than the HC MTT (p=0.0001) after 6 months on the respective diets, demonstrating greater insulin sensitivity and less stress on the B cells for insulin release for the same caloric intake with the HP diet

 β-hydroxybutyrate was measured as a determination of ketones on fasting blood during the study.  Both groups showed no significant difference in β-hydroxybutyrate from baseline to 6 months and no significant difference between groups and demonstrated no significant ketosis induced by the diets.  All chemistry profile and CBC parameters were in the normal range at baseline and 6 months. UUN increased significantly in the HP group from baseline to 6 months (8.9± 2.1 to 17.5 ± 2.3 gm/24 hr) compared to no increase in the HC group (8.8 ± 2.2 to 9.0 ± 1.6 gm/24 hr) verifying the HP group was consuming their HP diet.  PTH, 25-OH Vitamin D, CrCl, microalbumin, serum Ca or urinary Ca levels were not changed significantly (data not shown) in either the HP or HC group from baseline to 6 months.

Figure 2D: Shows the mean ± SE  of glucose and insulin for the 2 hour OGTTs and MTTs for the 6 HP diet subjects and the 6 HC diets subjects.  The colored lines represent the following: blue line is  HP diet baseline (HP_bl);   red line is HP diet at 6 months (HP_6m);   green line is HC diet baseline (HC_bl); and yellow line is HC diet at 6 months (HC_6m).

Figure 3 shows the percent lean mass(LM) and fat mass(FM) loss in the HP and HC groups. The HP group had a significant % increase in LM while there was a significant decrease in % FM from baseline to 6 months on the HP diet.  However, the HC group had a significant decrease in both the % LM and FM from baseline to 6 months on the HC diet, showing that the HP diet group had improvement in LM while achieving overall weight loss.

Figure 3: Shows the effect of the HP and HC diets on percent changes in lean body mass (LM)  and fat body mass (FM) at 6 months on the diets.

DISCUSSION

Important Findings

Important findings of this study which previously have not been reported are the following.

1) The HP diet resulted in 100% remission of Type 2 Diabetes to normal glucose tolerance in the subjects while the HC diet resulted in only 16 % remission in those subjects. To our knowledge this is the first lifestyle intervention study where remission of T2D has been studied with 100% remission with a feeding study using meals and food obtained from local grocery stores. This study shows that remission of T2D can be achieved with dietary modification if food intake parameters are tightly controlled. 

2) The HP diet group had greater improvement in insulin sensitivity and greater reduction in inflammation, oxidative stress (ROS) and cardiovascular risk factors than the HC group.

 3) The % lean body mass (LM) increased while % body fat mass (FM) was decreased in the HP diet group; whereas both % LM and FM decreased in the HC group. This preservation of % LM in the HP diet group may be an important factor in improving insulin sensitivity since muscle is a major insulin sensitive tissue for glucose uptake.

 4) A high level of compliance (>90%) was obtained by both the HP and HC diet groups by giving the diet meals and menus along with survey of food consumption to the subjects at weekly visits to our CRC.

Importance of Exercise:

Since the American Diabetes Association recommends that subjects with T2D exercise 30 minutes per day, all subjects were asked to walk for 30 minutes per day and this was monitored by Fitbits given to the subjects. There was no significant difference in amount of exercise between the HP and HC diet groups; therefore, not affecting the results.

Glucose and Insulin Changes Importance:

Although the OGTT glucose levels were similar at baseline, the HC group sustained significantly higher glucose levels remaining in the T2D range compared to the HP diet group which levels decreased to normal glucose levels after 6 months on the diet.  The HP MTT had significantly lower glucose levels at baseline and at 6 months than the HC MTT.  The increased improvement in insulin sensitivity and Beta cell function with the HP group compared to the HC group likely equates to decreased β cells stress in the HP group. Our study demonstrates that higher sustained glucose elevation with ingestion of glucose or higher glycemic foods as in the HC diet correlates with increased inflammation and oxidative stress in the HC group compared to the HP group. Antioxidant enzymes induced by repeated intake of excess energy in the form of high carbohydrate or high fat diets are not sufficient to block oxidative stress and inflammation has been shown (75). Therefore, the fact that our HP diet had a significantly greater reduction in inflammation, ROS, and cardiovascular risk factor markers than the HC diet in Type 2 Diabetes subjects is of great health significance.

Calcium Importance:

Our HP and HC diets contained 1500 mg Ca/day, more than the FDA recommended minimum amount of Ca /day (67) and showed no bone loss or loss of Ca in the urine (76). In contrast one study  reported that a HP diet caused negative calcium(Ca) balance with increased Ca loss in the urine which could indicate adverse effects in the bones (25).  However, our HP diet (30% protein) is at the upper limit of suggested protein consumption range (10-30%) which is not a sufficient percentage to cause a negative calcium (Ca) balance.

Ethnicity:

The majority of the subjects in our study were African American (AA) subjects and neither baseline HOMA-IR or ISI (Matsuda Index) were significantly different between the HP and HC groups; although possible limitation in assessment of insulin sensitivity by HOMA-IR with AA has been suggested (77). However, both methods of assessment showed greater improvement of insulin sensitivity with the HP diet than the HC diet at 6 months.

Diet Compliance:

Compliance of diet is an important factor in macronutrient composition diet studies. Strengths of this feeding study are that it was a randomized control trial where all food and daily diet plans were provided to each subject in both the HP and HC diet groups at their weekly visit to the CRC along with daily menu consumption survey each week for 6 months. This monitoring resulted in greater than 90 % diet adherence. A patient’s recall food questionnaire of food they ate days to weeks before is used in most studies which is generally inaccurate. Unlike weight loss counseling studies which rely solely on subject’s self determined food selections (DPP, Look Ahead) (6,78) and the liquid diet reported by Lean (79), we provided each subject with an individualized menu based on weight loss needs that map out what they are to eat each day for 6 months using foods obtained from local grocery stores. Our HP diet was 30% protein which is not excessive yet preserves muscle mass while not causing any liver or kidney problems; whereas, the high carbohydrate diet was 15% protein and resulted in loss of % lean body mass.

     Our study demonstrates that strict adherence of dietary intervention is possible and can produce meaningful and reliable results and remission of Type 2 Diabetes and demonstrates the importance of diet and exercise as also shown by others (80). Subjects that chose to stay on the HP diet plans for an additional 6 months purchasing their own food and consultations with the investigators when needed maintained their weight loss and normal glucose levels at the 6 months follow up visit.

The meal plans are adjustable to the subject’s food likes and dislikes with a wide variety of choices with different meal plans for each day of the week. All foods were commonly available at local grocery stores at a daily cost of around thirteen dollars. Initial instructions and weekly meal plans could be provided to the subject along with phone and email consultation when needed. The HP diet and instructional support used for this study would thus enable primary care physicians and other diabetes care givers to offer an economical means to achieve nutrition-based weight loss and remission of Type 2 Diabetes in both women and men.

Inflammation:

Type 2 diabetes is considered to be an inflammatory disease (81) and as we and others have shown these subjects have inflammatory cytokines as do subjects with ARDS, COVID-19 (82,83) and can exacerbate the inflammation response. Decreasing the inflammatory cytokines with remission of Type 2 Diabetes can help prevent other inflammatory diseases from becoming a cytokine storm of inflammation.

Our HP diet, although isocaloric with the HC diet, demonstrated profound metabolic improvements compared to the HC diet. This study shows that changing the macronutrients can improve insulin action by attenuating inflammation. This study is unique in that we used a non-pharmaceutical means (HP diet) as we have used in our previous studies of prediabetes remission, to achieve remission of T2D, weight loss, decreased inflammation, oxidative stress and cardiovascular risk factors

Funding Statement:

The study was funded by the American Diabetes Association (7-12-CT-41) and the

A.D. Baskin Research Fund (PI F. Stentz )

Competing Interests Statement:

 The authors have no competing interests to declare.

 Contribution Statement:

Author contributions: F.B.S. wrote the manuscript, researched data and contributed to the conception, design, coordinated recruitment and following subjects on the study, C.S. conducted history and physical examinations of subjects, reviewed data and manuscript. D.L., S.T., and J.C. performed some of the laboratory assays, reviewed and edited manuscript and organized patient data. Nutritionists at the UTHSC CRC provided information on diets and provided daily food and menus to participants, Dr. Jim Wan was the biostatistician in charge of statistical analysis.   F.B.S. is the guarantor of this work and, as such, had full access to the data in the study and take responsibility for the integrity of the data and accuracy of the data analysis. The manuscript has been read and approved by all the authors.

Acknowledgements:

The authors thank A. Ammons at UTHSC for assays of hormones and cytokines, all of the nursing and recruiting staff of the Clinical Research Center unit, all of whom are affiliated with the UTHSC, for their efforts and for assisting with some of the OGTT and MTTs.. The authors also thank all the study volunteers for their participation in the study. The Medical Student Research Fellowship Program from the National Institutes of Health/National Institute of Diabetes and Digestive Diseases (C5T35DK007405-28) (S. Dagogo-Jack, PI) at the University of Tennessee Health Science Center funded participation of second year medical students Damon Lawson and Sidney Tucker of this research study.
Data Sharing Statement:

No additional data are available.

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